Device for performing qualitative enzyme immunoassays

A device for performing an enzyme immunoassay, comprising:

• a body defining a receptacle including a first syringe containing a test liquid, and defining a second and a third syringe formed integrally in the body, the receptacle and syringes all having outlets;
• at least one assay tube being provided in the body, the tube having first and second ends and having an antibody, antigen or hapten attached to an internal surface of the tube;
• the body being provided with a flat bottom surface having grooves in it which connect outlets of the first, second and third syringes to the first end of the assay tube;
• a channel forming means being inserted over the bottom surface of the body, the means having a flat surface which coacts with the grooves to form channels for the flow of liquids from the syringes to the first end of the assay tube with the outlet of the second syringe being connected by a channel to the first end of the assay tube via the outlet of the first syringe;
• and
• the second and third syringes being each provided with first and second pistons spaced from one another, the second piston being directly depressed by a plunger, and each second and third syringe having a wall with a groove in it connecting the syringe outlet with the space above the first piston when it is fully depressed, the second syringe being provided with a first wash solution in the space defined therein below its first piston, and an enzyme conjugate containing solution in the space defined therein above its first piston;
• and the third syringe being provided with a second wash solution in the space defined therein below its first piston, and a solution containing enzyme substrate and enzyme metabolite indicator in the space defined therein above its first piston.