Feline leukemia virus vaccine
AssigneeCetus Corporation
InventorJack H. Nunberg
Life of this patent
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Abstract
Polypeptides that are synthesized or expressed directly in microorganisms, include an amino acid sequence that is homologous to at least a portion of gp70 envelope protein of FELV, and are immunogens that elicit or prime a humoral response in cats and immunize cats against FeLV infection. The microbially produced polypeptides are exemplified by a group of E. coli-expressed fusion proteins that comprise a portion of the trp leader peptide and a portion of the trp E protein fused to various portions of a subgroup B gp70 envelope protein. The synthesized polypeptides are exemplified by polypeptides that are homologous to segments of the hydrophilic domains of the gp70 protein.
Claims13
A microbially produced polypeptide comprising an amino acid sequence that is homologous to at least a portion of the amino acid sequence of gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and the 415-450 region of the FeLV gp85 protein, and is an immunogen that primes or elicits a humoral response in cats and is useful for immunizing cats against feline leukemia virus infection.
An E. coli produced fusion protein comprising:
- (a) a trpLE', fusion peptide sequence and
- (b) an amino acid seqeunce that is homologous to at least a portion of the amino acid sequence of gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and the 415-450 region of the FeLV gp85 protein, said fusion protein being an immunogen that primes or elicits a humoral response in cats and is useful for immunizing cats.
A conjugate of:
- (a) a polypeptide having an amino acid sequence that is homologous to at least a substantial portion of at least one of the hydrophilic domains of the gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and in the 415-450 region coupled to
- (b) a carrier protein.
A polypeptide of the formula
- (a) H - cys asp lys thr val arg leu arg arg glu pro ile ser leu - OH;
- (b) H - cys pro glu tyr val tyr thr his phe asp lys thr val arg leu - OH;
- (c) H - cys met gly pro asn leu val leu pro asp gln lys pro pro ser - OH;
- or
- (d) H - asp gln lys pro pro ser arg glu ser gln ile glu cys ser - OH.
Dependencies are inferred from the claim text.