Double-stranded DNA having sequences complementary to a single-stranded DNA and derived from mungbean yellow mosaic virus

An isolated, substantially pure double-stranded DNA, which is not digestible with restriction endonuclease Mbo I, having sequences complementary to a single-stranded DNA which has a molecular size of about 2.67 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 1 of the accompanying drawings.

An isolated, substantially pure double-stranded DNA, which is not digestible with restriction endonuclease DPNI, having sequences complementary to a single-stranded DNA which has a molecular size of about 2.70 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 2 of the accompanying drawings.

An isolated, substantially pure double-stranded DNA, which is not digestible with restriction endonuclease Mbo I, having sequences complementary to a single-stranded DNA which has a molecular size of about 2.70 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavae map shown in FIG. 2 of the accompanying drawings.

A hybrid DNA consisting essentially of a host vector, and inserted into said host vector DNA, an isolated substantially pure double-stranded DNA which is digestible with the restriction endonuclease Mbo I, but is not digestible with restriction endonuclease Dpn I, having sequences complementary to a single-stranded DNA which has a molecular size of about 2.67 Kb and is derived form mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 1 of the accompanying drawings.

A hybrid DNA consisting essentially of a host vector DNA, and inserted into said vector DNA, an isolated substantially pure double-stranded DNA, which is digestible with the restriction endonuclease Dpn I, but is not digestible with restriction endonuclease Mbo I, having sequences complementary to a single-stranded DNA which has a molecular size of about 2.67 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 1 of the accompanying drawings.

A hybrid DNA consisting essentially of a host vector DNA, and inserted into said host vector DNA, an isolated, substantially pure double-stranded DNA, which is digestible with the restriction endonuclease Mbo I, but is not digestible with restriction endonuclease Dpn I, having sequences complementary to a single-stranded DNA which has a molecular size of about 2.70 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 2 of the accompanying drawings.

A hybrid DNA consisting essentially of a host vector DNA, and inserted into said host vector DNA, an isolated, substantially pure double-stranded DNA, which is digestible with the restriction endonuclease Dpn I, but is not digestible with restriction endonuclease Mbo I, having sequences complementary to a single-stranded DNA which has a molecular size of about 270 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 2 or the accompanying drawings.