Biocompatible polyorganosiloxane composition for cell culture apparatus
OwnerAlbert J. Banesinventor
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Abstract
A polyorganosiloxane composition having a biocompatible surface thereon is disclosed. The biocompatible surface results from the derivatization, or amination, of the surface intended for cell contact. More specifically, the present invention is a polyorganosiloxane composition in which the surface is either treated with a primary amine and optional peptide or the surface is co-cured with a primary amine-containing silane or siloxane. The aminated polyorganosiloxane has utility as a cell culture substrate or in a variety of artificial organ applications such as brest implants, synthetic blood vessels, joints, tendons and heart valves. A vacuum apparatus for use with specialized cell culture plates incorporating the biocompatible polyorganosiloxane composition is also disclosed.
Claims25
A biocompatible resin, comprising:
- a polyorganosiloxane composition having incorporated at the surface thereof a substance selected from the group consisting of an amine, a carboxylic acid, or elemental carbon.
A biocompatible resin, comprising:
- a polyorganosiloxane composition having co-cured at the surface thereof a composition selected from the group consisting of primary amine-containing silanes, carboxyl-containing silanes, primary amine-containing siloxanes and carboxyl-containing siloxanes.
A cell culture substrate comprising:
- solid means for supporting a cell culture having at least one surface further comprising a polyorganosiloxane composition having incorporated at the surface thereof a substance selected from the group consisting of an amine, a carboxylic acid, or elemental carbon.
A cell culture substrate comprising:
- solid means for supporting a cell culture having at least one surface further comprising a polyorganosiloxane composition having a surface treated by co-curing therewith an adjacent layer containing a compound selected from the group consisting of primary amine-containing silanes, carboxyl-containing silanes, primary amine-containing siloxanes and carboxyl-containing siloxanes.
A cell culture substrate comprising a cell culture plate having one or more wells therein, each of said wells having a substantially planar base formed at least partially of an elastomeric membrane, with said elastomeric membrane having an upper surface treated to permit cell growth and attachment thereto by means of the incorporation at said upper surface of a substance selected from the group consisting of an amine, a carboxylic acid, or elemental carbon.
Dependencies are inferred from the claim text.