Method and apparatus for measuring the degree of reaction between a foreign entity and a subject's blood cells
OwnerMark J. Pasulainventor
Life of this patent
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Abstract
The invention is directed to a method for the diagnosis of a malady in a subject by observing a degree of reaction between all blood cells: red blood cells, leukocytes and platelets; in the subject's blood with a foreign entity having a predetermined relationship with the malady being diagnosed. The test includes comparing amounts and sizes of red blood cells, leukocytes and/or platelets in a control sample and at least one test sample. The test sample includes a portion of the subject's blood and the foreign entity being tested.
Claims18
A method for diagnosing the presence or absence of an immune response indicative of a malady in a subject, said method comprising the steps of:
- counting a number of blood cells of a single type selected from the group consisting of red blood cells and platelets, in a first blood sample drawn from said subject;
- mixing a substance having a predetermined relationship with said malady and eliciting a cellular immune response with a second blood sample drawn from said subject to form a mixture, and thereby allow blood cells of said second blood sample to react with said substance;
- counting a number of unreacted and reacted blood cells of said single type in said mixture;
- and
- comparing said number of said blood cells counted in said first blood sample with said number of unreacted and reacted blood cells counted in said mixture, to determine if said blood cells in said second blood sample reacted with said substance, thereby diagnosing the presence or absence of said malady in said subject.
A method for diagnosing the presence or absence of an immune response indicative of a malady in a subject having blood, said blood including blood cells of at least one of red blood cells, leukocytes and platelets, said method comprising the steps of:
- separating a sample of said blood of said subject into a control sample and at least one test sample, each of said control sample and said at least one test sample having approximately equal distributions of blood cells therein;
- counting a number of a first type of blood cells in said control sample;
- lysing at least a portion of said blood cells in said control sample;
- counting a number of a second type of blood cells in said control sample;
- mixing a substance with a predetermined relationship to said malady and eliciting a cellular immune response with said at least one test sample, thereby producing a mixture;
- counting a number of a first type of blood cells in said mixture;
- comparing said number of said first type of blood cells in said mixture with said number of said first type of blood cells in said control sample, thereby producing a first result;
- lysing a portion of said blood cells in said mixture;
- counting a number of a second type of blood cells in said test sample;
- and
- comparing said number of said second type of blood cells in said mixture with said number of said second type of blood cells in said control sample, thereby producing a second result, whereby the presence or absence of said malady may be determined by said first and second results.
A method for diagnosing the presence or absence of an immune response indicative of a malady in a subject having blood, said blood including blood cells of at least one of red blood cells, leukocytes and platelets, said method comprising the steps of:
- separating a sample of said blood of said subject into a control sample and at least one test sample, each of said control sample and said at least one test sample having approximately equal distributions of blood cells therein;
- counting a number of platelets in said control sample;
- counting a number of red blood cells in said control sample;
- counting a number of leukocytes in said control sample;
- mixing a substance with a predetermined relationship to said malady and eliciting a cellular immune response with said at least one test sample, thereby producing a mixture;
- counting a number of platelets in said mixture;
- comparing said number of platelets in said mixture with said number of platelets in said control sample, thereby producing a first result;
- counting a number of red blood cells in said mixture;
- comparing said number of red blood cells in said mixture with said number of red blood cells in said control sample, thereby producing a second result;
- counting a number of leukocytes in said mixture;
- comparing said number of leukocytes in said mixture with said number of leukocytes in said control sample, thereby producing a third result, whereby the presence or absence of said malady may be determined by said first, second and third results.
Dependencies are inferred from the claim text.