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US 4,788,148 AGranted
Plasmid with stabilized inheritance
AssigneeA C Biotechnics AB
InventorsLars A. J. Nilsson, Stig G. Skogman
Life of this patent
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Abstract
A plasmid with stabilized inheritance containing at least one production gene introduced colinearly by gene technology. The plasmid is characterized by a nucleic acid fragment colinearly introduced therein and constituted by the gene for valyl-tRNA synthetase; cell containing such plasmid; and a process for preparing the plasmid.
Claims6
A recombinant plasmid of stabilized inheritance, which comprises:
- a first nucleic acid fragment containing a production gene, said gene not normally found in the plasmid and encoding a polypeptide, and a second nucleic acid fragment containing a gene encoding valyl-tRNA synthetase, said first and second fragments each being situated in said plasmid so that each is operational, wherein said plasmid is one which is derived from an E. coli microorganism and wherein the plasmid containing said first and second nucleic acid fragments exhibits stabilized inheritance upon transformation and growth in a host E. coli microorganism due to expression of both the production gene and the valyl-tRNA synthetase gene.
Dependencies are inferred from the claim text.