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US 4,788,143 AGranted
Method for determining levels of cell-mediated immunity
OwnerAlexander A. Yabrov, Theodore Khavkininventors
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Abstract
A patient's level of cell-mediated immunity is determined by obtaining a blood sample from the patient, incubating cells from the sample in a tissue culture medium containing Concanavalin A, and observing the number of polykaryons produced or the number of nuclei per polykaryon or both to thereby determine the level of cell-mediated immunity of the hose from which the blood sample was obtained.
Claims12
A method for determining levels of cell-mediated immunity by assaying autologous cells, comprising the steps of:
- obtaining a whole blood sample from a host;
- incubating cells comprising monocytes from the sample in a tissue culture medium containing Concanavalin A for between 18 and 48 hours;
- and
- observing the number of short-lived monocyte polykaryons produced or the increase in number of nuclei per polykaryon or both to thereby determine the level of cell-mediated immunity of the host from which the blood sample was obtained.
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Dependencies are inferred from the claim text.