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AssigneeBeckman Instruments, Inc.
InventorsKer-Kong Tung, Linda K. Cragle, Frederick W. Rood, Jr., Shih-Yun Lee
Estimating the term…
A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody:antigen:labelled antibody complex which is then detected. Unbound unlabelled first antibody and/or unbound unlabelled second antibody are added to the reaction mixture to divert excess antigen away from the desired end-product complex, thus rendering the antigen of interest the rate-limiting factor in the overall immunoreaction. This results in a pseudo first-order reaction which produces an actual linear standard curve.
A method for performing an immunoassay for an antigen in a liquid sample comprising the steps of forming a complex between the antigen and at least two antibodies, one of said antibodies being a first antibody bound to an insoluble support and one of said antibodies being a second unbound labelled antibody, whereby a portion of said labelled antibody becomes bound to the insoluble support through said complex, and introducing thereto additional antibody selected from the group consisting of unlabelled unbound first antibody, unlabelled unbound second antibody, and mixtures thereof.
A method of achieving a linear standard curve in an immunoassay for the determination of an antigen in a liquid sample comprising the steps of:
A reagent comprising:
A method of immunoassay for an antigen in a liquid sample comprising the steps of forming a complex between antigen contained in the sample and at least two antibodies, one of said antibodies being a first antibody bound to an insoluble support and one of said antibodies being a second unbound labelled antibody, whereby a portion of said labelled antibody becomes bound to the insoluble support through said complex, and adding thereto an unlabelled unbound analogue for said first antibody.
A method of immunoassay for an antigen in a liquid sample comprising the steps of forming a complex between antigen contained in the sample and at least two antibodies, one of said antibodies being a first antibody bound to an insoluble support and one of said antibodies being a second unbound labelled antibody, whereby a portion of said labelled antibody becomes bound to the insoluble support through said complex, and adding thereto an unlabelled unbound analogue for said second antibody.
A method of immunoassay for an antigen in a liquid sample comprising the steps of forming a complex between antigen contained in the sample and at least two antibodies, one of said antibodies being a first antibody bound to an insoluble support and one of said antibodies being a second unbound labelled antibody, whereby a portion of said labelled antibody becomes bound to the insoluble support through said complex, and adding thereto unlabelled unbound analogues for said first and second antibodies.
Dependencies are inferred from the claim text.