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US 4,787,971 AGranted
Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
OwnerAlan Donaldinventor
Life of this patent
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Abstract
The present invention is directed to providing a column chromatography apparatus and assay method employing the same which is readily adaptable for rapid laboratory separation of chemicals, in particular, organic, inorganic and biochemicals and biomolecules from an eluent fluid, such as a body fluid. In particular, it has been determined that a miniaturized column chromatography apparatus having a multi-stage separatory tube, an associated eluent tube, an adapter and a vented-cap and a non-vented cap is particularly well suited to the rapid assays yielding accurate results which are required in the analytical laboratory or clinical setting.
Claims24
A miniaturized column chromatography apparatus, comprising:
- a generally tubular column member having a plurality of chambers, each of said plurality of chambers being disposed in fluid flow communication with another of said plurality of chambers, said tubular column member further having an eluent inlet and eluate outlet, said inlet having a relatively larger diameter than and in fluid flow communication with said eluate outlet, said eluate outlet further having a generally frusto-conical shape;
- a planar disc-shaped porous filter horizontally disposed within said generally tubular column member and subtending said eluate outlet;
- chromatographic separatory medium packed within said generally tubular column member, said chromatographic separatory medium being selected dependent upon the materials to be separated;
- vented capping means for closing said eluate inlet disposed within eluate inlet opening and comprising a cap and a cap adapter, wherein said cap further comprises a generally cylindrical hollow lower stalk portion and a generally planar upper cap portion having a lower ring portion depending therefrom, wherein said lower ring portion has an inside diameter corresponding to an outside diameter of an upper rim portion of said generally tubular column member;
- and wherein said cap adapter further comprises a generally cylindrical hollow upper portion, a generally cylindrical hollow lower portion depending therefrom and a radial flange extending outwardly from an upper surface of said generally cylindrical lower portion, said upper portion having outside diameter corresponding to an inner diameter of said generally cylindrical hollow lower stalk portion of said cap and said lower portion having an outside diameter corresponding to an inside diameter of an upper portion of said generally tubular column member;
- and
- an eluate receiving member having a rounded bottom portion for forming a pellet therein;
- said eluate receiving member being disposed in fluid communication with said generally tubular column member.
5 more elements
A miniaturized column chromatography apparatus, comprising, in combination:
- a multi-stage column member having an eluate inlet and eluent outlet opening and a plurality of chambers in fluid flow communication disposed therebetween, each of said chambers having an inlet and outlet opening, each of said inlet openings further having a relatively larger diameter than a communicating outlet opening, said eluate outlet further having a generally frustro-conical shape and a central bore passing therethrough;
- at least one of a plurality of porous polymeric disc-shaped filters horizontally disposed within said multi-stage column member and subtending said eluate outlet and at least one of said outlet openings of said plurality of chambers;
- at least one of a plurality of chromatographic separatory media packed within said generally tubular generally tubular column member, said chromatographic separatory media being selected dependent upon the materials to be separated wherein a different and distinct one of said at least one of a plurality of chromatographic separatory media is packed in each of said plurality of chambers of said multi-stage column member for separating different and distinct materials from a solution;
- a cap member having a generally cylindrical hollow lower stalk portion and a generally planar upper cap portion having a lower ring portion depending therefrom, wherein said lower ring portion has an inside diameter corresponding to an outside diameter of an upper rim portion of said generally tubular column member;
- a cap adapter having a generally cylindrical hollow upper portion, a generally cylindrical hollow lower portion depending therefrom and a radial flange extending outwardly from an upper surface of said generally cylindrical lower portion, said upper portion having outside diameter corresponding to an inner diameter of said generally cylindrical hollow lower stalk portion of said cap and said lower portion having an outside diameter corresponding to an inside diameter of an upper portion of said generally tubular column member;
- and
- an eluate receiving member disposed in fluid communication with said generally tubular column member.
4 more elements
Dependencies are inferred from the claim text.