Factor IX therapeutic blood product, means and methods of preparing same
AssigneeBlood Systems, Inc.
InventorKenneth J. Smith
Life of this patent
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Abstract
A method of producing highly purified factor IX from crude factor IX comprising binding an A-7 monoclonal antibody to an Affigel-10 column; partially purifying the crude factor IX to produce a partially purified factor IX; applying that factor IX to the A-7-Affigel-10 column with an application buffer; washing the column with 1-3M NaCl in 0.05M Tris-HC1, 20 mM MgCl.sub.2 and 1 mM Benzamidine HCl; eluting purified factor IX from the washed column with 20 mM EDTA; and separating purified factor IX from the EDTA. An improved factor IX product produced thereby.
Claims11
The method of producing highly purified factor IX from crude factor IX comprising the steps of:
- (a) binding an A-7 monoclonal antibody to an Affigel-10 column;
- (b) partially purifying said crude factor IX to produce a partially purified factor IX;
- (c) applying said partially purified factor IX to said Affigel-10 column having said A-7 antibody bound thereto with an application buffer;
- (d) washing said column with 1M-3M NaCl in 0.05M Tris-HCl, 20 mM MgCl.sub.2 and 1 mM Benzamidine HCl;
- (e) eluting purified factor IX from said washed column with 20 mM EDTA;
- and
- (f) separating said purified factor IX from said EDTA.
Dependencies are inferred from the claim text.