Analytical method and element for protein assay
AssigneeEastman Kodak Company
InventorsJohn C. Mauck, Harold C. Warren, III
Life of this patent
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Abstract
There is disclosed a method for quantitatively determining protein, comprising the steps of: (a) providing a sample of the protein in an aqueous medium; (b) providing an aqueous medium having a pH in excess of 12 and comprising (i) a cupric salt and a pyridyl-azo dye; or (ii) a preformed cupric-pyridylazo dye complex; (c) combining the aqueous mediums of a) and b) thereby providing a color having an intensity which is inversely proportional to the amount of unreacted dye present in the combined mediums; and (d) determining the quantity of protein present in the sample colorimetrically. The method can be used with a multilayer dry analytical element.
Claims15
A method for quantitatively determining protein, comprising the steps of:
- (a) providing a sample of the protein in an aqueous medium;
- (b) providing an aqueous medium having a pH in excess of 12 and comprising
- (i) a cupric salt and a pyridyl-azo dye;
- or
- (ii) a preformed cupric-pyridyl-azo dye complex;
- (c) combining the aqueous mediums of (a) and (b) thereby forming a color having an intensity which is inversely proportional to the amount of unreacted dye present in the combined mediums;
- and
- (d) determining the quantity of protein present in the sample colorimetrically.
An analytical element for determination of protein in an aqueous medium comprising an absorbent material which contains
- (i) a cupric salt and a pyridyl-azo dye or a preformed cupric-pyridyl-azo dye complex and
- (ii) a base composition capable of establishing a pH in excess of 12 when the absorbent material is contacted with an aqueous medium.
A multilayer analytical element for determination of protein in an aqueous medium, comprising
- (i) a support bearing,
- (ii) a spreading layer,
- (iii) one or two reagent layers in fluid contact with the spreading layer;
- and
- (iv) a cupric salt, a pyridyl-azo dye or a preformed cupric-pyridyl-azo dye complex and a base composition capable of establishing a pH in excess of 12;
- wherein
- the salt and the dye are in the same or different layers.
A method for the quantitative analysis of proteins in an aqueous liquid sample which comprises
- (a) providing an analytical element for the determination of protein in an aqueous medium comprising an absorbent material which contains
- (i) a support bearing,
- (ii) a spreading layer,
- (iii) a reagent layer in fluid contact with the spreading layer, and comprising a cupric salt and a pyridyl-azo dye or a preformedd cupric-pyridyl-azo dye complex;
- and
- (b) applying a portion of the sample to the spreading layer of the analytical element,
- (c) colorimetrically determining, after a predetermined time, the quantity of protein in the sample wherein the color intensity is inversely proportional to the amount of unreacted dye.
Dependencies are inferred from the claim text.