Method for separating plasma from blood and silanized glass fibres therefor
AssigneeBoehringer Mannheim GmbH
InventorsHans Wielinger, Helmut Freitag
Life of this patent
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Abstract
Hydrophilic glass fibers which have bonded to their surfaces a layer of silane molecules of the formula ##STR1## are useful for the separation of plasma from blood for haemostasiological testing. In the formula: the molecule is bonded at its end oxygen to a surface of a glass fibre; R.sub.1 is C.sub.2 -C.sub.6 alkylene; X is OH, C.sub.1 -C.sub.6 alkoxy, having n carbon atoms and up to (n-1) OH groups in which n is 1-6, or an amino acid or peptide residue; and each of R'.sub.3 and R".sub.3 is C.sub.1 -C.sub.6 alkyl, an oxygen bridge to the silicon atom of another silane molecule or an oxygen bridge to the glass surface. The fibres have an average diameter of from 0.2 to 5 microns and a density of 0.1 to 0.5 g/cm.sup.3.
Claims14
An agent for separating plasma from blood for haemostasiological tests which comprises a layer of glass fibres having bonded to their surfaces a layer of silane molecules of the formula ##STR4## wherein each molecule is bonded at its end oxygen to a surface of a glass fibre in the layer of glass fibres,
- R.sub.1 is C.sub.2 -C.sub.6 alkylene,
- X is hydroxyl, C.sub.1 -C.sub.6 alkoxy, an alkoxy radical having n carbon atoms and up to (n-1) hydroxyl groups in which n is an integer from 1 to 6, or an amino acid or peptide residue;
- and each of R'.sub.3 and R".sub.3 is C.sub.1 -C.sub.6 alkyl, an oxygen bridge to the silicon atom of another silane molecule, or an oxygen bridge to a glass surface,
- said fibres having an average diameter of from 0.2 to 5.mu. and said glass fiber layer having density of 0.1 to 0.5 g./cm.sup.3.
A method for separating plasma from blood which comprises passing whole blood through an agent comprising a layer of glass fibres having bonded to their surfaces a layer of silane molecules of the formula ##STR5## wherein each molecule is bonded at its end oxygen to a surface of a glass fibre in the layer of glass fibres,
- R.sub.1 is C.sub.2 -C.sub.6 alkylene,
- X is hydroxyl, C.sub.1 -C.sub.6 alkoxy, an alkoxy radical having n carbon atoms and up to (n-1) hydroxyl groups in which n is an integer from 1 to 6, or an amino acid or peptide residue;
- and
- each of R'.sub.3 and R".sub.3 is C.sub.1 -C.sub.6 alkyl, an oxygen bridge to the silicon atom of another silane molecule, or an oxygen bridge to a glass surface, said fibres having an average diameter of from 0.2 to 5.mu. and said glass fibre layer having a density of 0.1 to 0.5 g./cm.sup.3.
Dependencies are inferred from the claim text.