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US 4,786,599 AGranted
Serum-free animal cell culture medium and methods for the primary culture and production of cell lines using this medium
AssigneeInstitut National de la Sante et de la Recherche Medicale
InventorsMartina L. Chessebeuf, Prudent H. Padieu
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Abstract
A serum-free animal tissue culture medium contains a mixture of six fatty acids and albumin or dextran. The medium is particularly adapted for the primary culture of rat liver epithelial cells and possibly in the presence of hormones and/or growth factors, for obtaining cell lines, in particular of myelomae and hybridomae.
Claims20
Serum-free animal cell culture medium, characterized in that is consists essentially of:
- (a) a synthetic basal medium designed for animal cell culture;
- (b) 0.05 to 30 .mu.eq/l of a mixture of the six fatty acids palmitic acid, cis-palmitoleic acid, stearic acid, cis-oleic acid, cis-linoleic acid and cis-linolenic acid or their esters, in the following proportions, relative to the free fatty acid:
- from 0.0155 to 24.0 .mu.mole/l of palmitic acid;
- from 0.0058 to 9.0 .mu.mole/l of cis-palmitoleic acid;
- from 0.0014 to 2.0 .mu.mole/l of stearic acid;
- from 0.0067 to 10.0 .mu.mole/l of cis-oleic acid;
- from 0.0178 to 27.0 .mu.mole/l of cis-linolenic acid;
- and
- from 0.0028 to 4.0 .mu.mole/l of cis-linolenic acid, adsorbed on
- (c) at least one water-soluble lipophile biopolymer present in an amount capable of ensuring the dissolution of the fatty acids or esters present in the medium.
7 more elements
Dependencies are inferred from the claim text.