Process for determining the binding capacity of thyroxin-binding globulin
AssigneeBoehringer Mannheim GmbH
InventorsBrigitte Draeger, Winfried Albert, Martina Junius, Jurgen Rasch
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Abstract
The present invention provides a process for determining the binding capacity of thyroxin-binding globulin (TBG) according to the principle of heterogeneous enzyme immunoassay, wherein the serum sample to be investigated is incubated with enzyme-labelled T.sub.4 or T.sub.3 and immobilized antibody against TBG or T.sub.4, the phase are separated and the labelling enzyme is measured in one of the phases.
Claims13
A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T.sub.4 or T.sub.3 bound via a spacer to an enzyme label and immobilized antibody which specifically binds to TBG under conditions favoring binding of TBG to labeled T.sub.4 or T.sub.3 and formation of complexes between said immobilized antibody and TBG bound to labeled T.sub.4 or T.sub.3, separating free and immobilized phrases and measuring label in one of the phases as a measurement of the binding capacity of thyroxin binding globulin in said sample.
A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T.sub.4 bound via a spacer to an enzyme label without addition of unlabeled T.sub.4 and with immobilized antibody which specifically binds to labeled T.sub.4 unbound to TBG under conditions favoring binding of TBG to labeled T.sub.4 and formation of complexes between labeled T.sub.4 and said immobilized antibody, separating free and immobilized phases and measuring label in one of said phases as a measurement of the binding capacity of thyroxin binding globulin in said sample.
A process for determining the binding capacity of thyroxin binding globulin (TBG) with T.sub.4 or T.sub.3 bound via a spacer to an enzyme, an antibody specific for TBG and an immobilized antibody which specifically binds to the Fc portion of said TBG specific antibody under conditions favoring binding of TBG to said labeled T.sub.4 or T.sub.3, said TBG specific antibody to TBG, and said immobilized Fc specific antibody to said TBG specific antibody, separating free and immobilized phases and measuring the label in one of said phases as a measure of the binding capacity of thyroxin binding globulin in said sample.
A process for determining the binding capacity of thyroxin binding globulin (TBG) comprising incubating a serum sample containing TBG with T.sub.4 bound via a spacer to an enzyme, an antibody specific for labeled T.sub.4 and an immobilized antibody which specifically binds to the Fc portion of said T.sub.4 specific antibody under conditions favoring binding of T.sub.4 to TBG, T.sub.4 to said T.sub.4 specific antibody and said immobilized Fc specific antibody to said T.sub.4 specific antibody, separating free and immobilized phases and measuring the label in one of said phases as a measure of the binding capacity of thyroxin binding globulin in said sample.
Dependencies are inferred from the claim text.