Process for the promotion of regeneration of plants from tissue cultures
AssigneeRichter Gedeon Vegyeszeti Gyar Rt.
InventorsPeter Medgyesy, Laszlo Marton, Laszlo Purhauser
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Abstract
The invention relates to a process for the promotion of regeneration of differentiated plants from vegetable cells maintained in tissue cultures by adding inhibitors of ethylene production or ethylene action to the hormone-containing or hormone-free culture medium used for maintaining the tissue cultures. The process is equally suitable for the promotion of the regeneration of mono- and dicotyledons. As an inhibitor preferably silver nitrate or cobalt chloride is employed.
Claims9
A process for inducing or increasing shoot regeneration of a cereal or tobacco plant from plant cells cultured in a tissue culture medium which comprises the steps of:
- (a) providing a tissue culture medium comprising a plant hormone and 5 to 100 mg/liter of silver nitrate;
- and
- (b) forming a tissue culture by cultivating cereal or tobacco plant cells in the tissue culture medium whereby the silver nitrate inhibits ethylene action thereby inducing or increasing shoot regeneration.
A process for inducing or increasing shoot regeneration of a cereal or tobacco plant from plant cells cultured in a tissue culture medium which comprises the steps of:
- (a) providing a tissue culture medium comprising a plant hormone;
- (b) forming a tissue culture by cultivating cereal or tobacco plant cells in the tissue culture medium comprising a plant hormone;
- (c) removing the cultivated cereal or tobacco plant cells from the tissue culture medium comprising a plant hormone;
- (d) transferring the cultivated cereal or tobacco plant cells to a hormone-free culture medium containing 5 to 100 mg/l silver nitrate;
- and
- (e) further cultivating the cereal or tobacco plant cells in the hormone-free tissue culture whereby the silver nitrate inhibits ethylene action thereby inducing or increasing shoot regeneration.
A process for inducing or increasing shoot regeneration of a cereal or tobacco plant from plant cells cultured in a tissue culture medium which comprises the steps of:
- (a) providing a tissue culture medium comprising 5 to 100 mg/liter of cobalt chloride;
- and
- (b) forming a tissue culture by cultivating cereal or tobacco plant cells in the tissue culture medium whereby the cobalt chloride inhibits ethylene production thereby inducing or increasing shoot regeneration.
A process for inducing or increasing shoot regeneration of a cereal or tobacco plant from plant cells cultured in a tissue culture medium which comprises the steps of:
- (a) providing a tissue culture medium comprising a plant hormone;
- (b) forming a tissue culture by cultivating cereal or tobacco plant cells in the tissue culture medium comprising a plant hormone;
- (c) removing the cultivated cereal or tobacco plant cells from the tissue culture medium comprising a plant hormone;
- (d) transferring the cultivated cereal or tobacco plant cells to a hormone-free culture medium containing 5 to 100 mg/l cobalt chloride;
- and
- (e) further cultivating the cereal or tobacco plant cells in the hormone-free tissue culture whereby the cobalt chloride inhibits ethylene production thereby inducing or increasing shoot regeneration.
Dependencies are inferred from the claim text.