Vector
AssigneeImperial Chemical Industries PLC
InventorNigel J. Grinter
Life of this patent
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Abstract
Novel transposable cloning vector and method of using it for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A) a part of whose DNA including the sequence which specifies its transposition function has been removed. The second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition function. The DNA material to be inserted into the chromosome of the recipient bacterium is included in the DNA of transposon (A) carried by the first plasmid. Any suitable transposon may be used as transposon (A) but transposon Tn7 is preferred.
Claims8
A vector for the transfer of DNA material between microorganisms which comprises a first plasmid and a second plasmid wherein the first plasmid carries a transposon (A), a part of whose DNA including the sequence which specifies its transposition function has been removed and the second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition function, transposon (A) being any suitable transposon.
A method for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material (B) foreign to that bacterium which comprises the steps of (1) constructing a vector-containing microorganism which contains a carrier plasmid and a helper plasmid, the carrier plasmid carrying any suitable transposon (A) into whose DNA has been included the foreign DNA material (B) to be inserted into the recipient bacterium and a part of whose DNA including the sequence specifying its transposition function has been removed and the helper plasmid carrying a fragment of DNA from a transposon (A) including the sequence specifying its transposition function, (2) transferring the carrier and the helper plasmids directly or indirectly from the vector-containing microorganism into the recipient bacterium and, (3) growing the recipient bacterium for sufficient generations and under suitable conditions for the inserted foreign DNA material (B) to pass into the chromosome of the recipient bacterium.
Dependencies are inferred from the claim text.