Immunogen conjugates and the use thereof in a dihydropyridine assay
AssigneeMiles Inc.
InventorKevin P. Campbell
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Abstract
Disclosed are 1,4-dihydropyridine immunogen conjugates of immunogenic carrier materials coupled to a 1,4-dihydropyridine derivative. Said conjugates are useful for the preparation of antibodies thereto which antibodies may be used in immunoassays for 1,4-dihydropyridine compounds. An affinity column useful for the purification of said antibodies is also disclosed.
Claims14
1,4-dihydropyridine immunogen conjugates of the formula:
- ##STR4## wherein R is bovine serum albumin, casein, ovalbumin or keyhole limpet hemocyanin.
An antibody prepared against a 1,4dihydropyridine immunogen conjugate of the formula:
- ##STR5## wherein R is a protein or polypeptide having a molecular weight of at least 4000.
A cell line having American Type Culture Collection accession number HB 8847.
A monoclonal antibody produced by a cell line having American Type Culture Collection accession number HB 8847.
A cell line having American Type Culture Collection accession number HB 8889.
A monoclonal antibody produced by a cell line having American Type Culture Collection accession number HB 8889.
A test kit for determining the presence of 1,4-dihydropyridines in a biological sample, which 1,4-dihydropyridines are capable of functioning as calcium channel blocking agents, which kit comprises at least one receptacle containing:
- (a) one or more of a radiolabeled 1,4-dihydropyridine;
- (b) an antibody specific for 1,4-dihydropyridines;
- and
- (c) serial dilutions of 1,4-dihydropyridine standard solutions.
Dependencies are inferred from the claim text.