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AssigneeZymoGenetics, Inc.
InventorsFrederick S. Hagen, Mark J. Murray, Sharon J. Busby, Kathleen L. Berkner, Margaret Y. Insley, Richard G. Woodbury, Charles L. Gray
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Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. In particular, the first nucleotide sequence may be derived from a genomic clone or cDNA clone of Factor VII. The second sequence encodes a catalytic domain for the serine protease activity of Factor VIIA. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as Factor VIIa.
A DNA construct comprising a DNA sequence encoding Factor VII.
A DNA construct comprising a first nucleotide sequence joined to a second nucleotide sequence positioned downstream of said first sequence, said first and second sequences derived from cDNA clones of Factor VII, the joined sequences coding for a protein which upon activation has substantially the same biological activity for blood coagulation as Factor VIIa.
A DNA construct comprising a first nucleotide sequence derived from a genomic clone of Factor VII, joined to a second nucleotide sequence positioned downstream of said first sequence, said second sequence derived from a cDNA clone of Factor VII, the joined sequence coding for a protein which upon activation has substantially the same biological activity for blood coagulation as Factor VIIa.
A recombinant plasmid comprising a DNA sequence encoding Factor VII.
A recombinant plasmid capable of integration in mammalian host cell DNA, said plasmid comprising a promoter followed downstream by a DNA sequence encoding Factor VII, said DNA sequence being followed downstream by a polyadenylation signal.
A recombinant plasmid capable of integration in mammalian host cell DNA, said plasmid comprising a promoter followed downstream by a first nucleotide sequence joined to a second nucleotide sequence positioned downstream of said first sequence, said first and second sequences derived from cDNA clones of Factor VII, the joined sequences coding for a protein which upon activation has substantially the same biological activity for blood coagulation as Factor VIIa, the joined sequences being followed downstream by a polyadenylation signal.
A recombinant plasmid capable of integration in mammalian host cell DNA, said plasmid comprising a promoter followed downstream by a first nucleotide sequence derived from a genomic clone of Factor VII, joined to a second nucleotide sequence positioned downstream of said first sequence, said second sequence derived from a cDNA clone of Factor VII, the joined sequences coding for a protein which upon activation has substantially the same biological activity for blood coagulation as Factor VIIa, the joined sequences being followed downstream by a polyadenylation signal.
Mammalian cells stably transfected with a recombinant plasmid comprising a DNA sequence encoding Factor VII, said cells producing Factor VII in recoverable amounts.
A method for producing a protein having biological activity for blood coagulation mediated by Factor VIIa, comprising:
Dependencies are inferred from the claim text.