Method for assaying the gamma-interferon productivity of blood
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorMasakazu Mitsuhashi
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Abstract
Production of HuIFN-gamma and assay of blood HuIFN-gamma productivity both using donated blood are disclosed. Human whole blood secrets a high titer of HuIFN-gamma when exposed to an IFN-gamma inducer (e.g. mitogen) in the presence of anticoagulant (e.g. heparin, ACD, and CPD). Blood HuIFN-gamma productivity is determined by titrating the secreted HuIFN-gamma with a suitable procedure (e.g. bioassay, radio-immunoassay, or enzyme-linked immunosorbent assay). The blood of cancer patient is lower in HuIFN-gamma productivity than that of healthy donor. The HuIFN-gamma per se is recovered and purified prior to its prophylactic and therapeutic uses.
Claims18
A clinical assay for detecting human cancer, said method comprising the steps of:
- (a) collecting fresh human whole blood from a patient suspected of having cancer;
- (b) incubating said human whole blood at a temperature in the range of 30-40.degree. C. for 10-90 hours while exposing it to an anticoagulant and a gamma-interferon inducer (IFN-gamma inducer);
- and
- (c) determining the level of HuIFN-gamma in the resultant culture
- (d) comparing said level of HuIFN-gamma against a standard determined by measuring the HuIFN-gamma productivity of whole blood collected from a healthy patient and incubated under the conditions of step (b)
- (e) whereby an HuIFN-gamma level obtained by said culturing of said blood of said suspected cancer patient which is significantly lower than said standard indicates the presence of cancer.
A clinical assay for detecting human cancer, comprising:
- (a) collecting whole blood from a subject;
- (b) removing the plasma from the donated whole blood;
- (c) suspending the residual solid in a member selected from the group consisting of saline, isotonic buffer, and a nutrient culture medium;
- (d) incubating in vitro a predetermined amount of the resultant cell suspension at temperature in the range of 30.degree.-40.degree. C. for 10-90 hours while exposing it to an anticoagulant and an interferon-gamma inducer;
- and
- (f) determining the interferon-gamma level in the resultant culture,
- (g) comparing said interferon-gamma level against a standard predetermined by measuring normal level of interferon-gamma obtained by collecting whole blood from a healthy patient and performing steps (b) through (f) upon the collected whole blood;
- (h) whereby a level of interferon gamma obtained by said processing of said blood of said suspected cancer patient according to steps (b) through (f) significantly below said standard indicates the presence of cancer.
Dependencies are inferred from the claim text.