Quantitation of cancer procoagulant activity in serum
AssigneeMesa Medical, Inc.
InventorStuart G. Gordon
Life of this patent
Estimating the term…
Abstract
Methods and techniques are described for detecting the presence of cancer procoagulant activity in blood serum. The method provides a means of distinguishing persons or animals having cancer from those who do not have cancer. The method involves techniques for processing serum such that the activity of cancer procoagulant can be measured while the activity of other coagulation enzymes in the serum is minimized or eliminated. Then the serum can be analyzed for cancer procoagulant activity.
Claims12
A method for detecting cancer procoagulant activity in blood serum comprising the steps of:
- (a) obtaining a blood sample of animal or human blood;
- (b) removing essentially all cellular material from said sample;
- (c) dissociating said cancer procoagulant from other proteins present in said sample;
- (d) denaturing said other proteins in said sample without denaturing said cancer procoagulant;
- (e) separating said other proteins from said cancer procoagulant;
- and
- (f) analyzing said sample for said cancer procoagulant by determining cancer procoagulant activity.
A method for detecting cancer procoagulant activity in blood serum, said method comprising the steps of:
- (a) obtaining a blood sample of animal or human blood;
- (b) removing essentially all cellular material from said sample;
- (c) adding to said sample a buffer solution containing DMSO;
- (d) incubating said sample at room tmperature;
- (e) adding to said sample an extraction mixture comprising aluminum hydroxide gel and potassium cyanide;
- (f) heating said sample to a temperature of about 55.degree. C. for a time sufficient to denature coagulation factors and anti-proteinase without denaturing said cancer procoagulant;
- (g) centrifuging said sample;
- and
- (h) analyzing the supernatant of said sample for said cancer procoagulant by determining cancer procoagulant activity.
Dependencies are inferred from the claim text.