Process for producing natural heparan sulphate and dermatan sulphate in substantially pure form, and their pharmaceutical use

A process for producing natural heparan sulphate and dermatan sulphate is substantially pure form from mixtures of proteoglycans of animal tissues from the aorta, myocardium and vascularized organs, characterized by the following stages:

• extracting the proteoglycans from said tissues in finely micronized form by treatment with a solution of a compound selected from the group consisting of urea, quanidine, thiourea and potassium thiocyanate;
• filtering and clarifying the solution, and partially eliminating the solution of a compound selected from the group consisting of urea, quanidine, thiourea and potassium thiocyanate;
• splitting the bond between the mucopolysaccharides and proteins;
• precipitating the proteins and filtering
• eliminating the nucleic acid traces;
• precipitating the mucopolysaccharides;
• fractionating the heparan sulphate and dermatan sulphate and purifying them.