Growth of hepatitus A virus in vero cells
AssigneeMerck & Co., Inc.
InventorsPhilip J. Provost, Paula A. Giesa, William J. McAleer
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Abstract
Hepatitus A virus is grown in Vero cells after passage in in vitro cell culture. Virus replication and continued passage in Vero cells requires extended incubation times, up to about four weeks, for early passages and incubation temperatures no higher than about 32.degree. C. Continued passage results in a significant decrease in incubation time and an increase in virus yield. Cultivation of hepatitus A virus in Vero cells meets the development requirements for an inactivated human vaccine.
Claims8
A method of growing hepatitis A virus in cell culture comprising growth of infective virus by passaging at temperatures no higher than 33.degree. C. in Vero cells that have been exposed to an inoculum containing hepatitis A virus until there is a sufficient concentration of virus for vaccine production.
Dependencies are inferred from the claim text.