Polyacrolein-type microspheres
AssigneeYeda Research and Development Co. Ltd.
InventorsShlomo Margel, Uzi Beitler
Life of this patent
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Abstract
Suspension polymerization of acrolein type compounds in the presence of appropriate surfactants results in the formation of microspheres in size ranging from 0.03 to 80 microns. Fluorescent and magnetic microspheres are obtained by carrying out the same polymerization in the presence of appropriate fluorochromic or ferrofluidic compounds, respectively. Hybrido-polyacrolein microspheres are obtained by grafting one type of such microspheres on another type. Immunomicrospheres were formed by binding covalently at physiological pH appropriate proteins to the microspheres. The immunomicrospheres can be used for various biological applications, such as specific markers for labeling cell surface receptors, for cell separation, for diagnostic purposes, etc.
Claims13
A polyacrolein-type microsphere produced by subjecting an aqueous mixture of a polymerizable acrolein-type monomer to polymerization in the presence of an effective amount of a suitable ionic surfactant which promotes uniform microsphere formation upon polymerization of the monomer so as to form a polyacrolein-type microsphere, the polymerization being effected at a pH between 8.0 and 13.7, and recovering the resulting microsphere and purifying the microsphere so recovered, the microsphere having an aldehyde content of about 2.9 mmol/gram of microspheres, a double-bond content of about 10.9 mmol/gram of microspheres and a carboxyl content of about 0.34 mmol/gram of microspheres.
A polyacrolein-type microsphere having a diameter from 0.03 to 80 microns produced by subjecting under an inert atmosphere and suitable conditions an aqueous mixture of a polymerizable acrolein-type monomer to polymerization in the presence of an effective amount of a suitable surfactant which promotes uniform microsphere formation upon polymerization of the monomer, an oxidizing agent and reducing agent, the oxidizing agent and reducing agent both being present in amounts capable of initiating a redox reaction so as to effect polymerization of the acrolein-type monomer and microsphere formation, recovering the resulting microsphere and purifying the microsphere so recovered, the microsphere having an aldehyde content of about 12.0 mmol/gram of microspheres and a double-bond content of about 1.6 mmol/gram of microspheres.
Dependencies are inferred from the claim text.