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US 4,782,025 AGranted
Novel microorganism
AssigneeKao Corporation
InventorsShigeo Inoue, Yoshiharu Kimura
Life of this patent
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Abstract
A novel microorganism Torulopsis bombicola KSM-36 (FERM BP-799) isolated from cabbage leaves grown in a cabbage field located in Musashino-shi, Tokyo, has sophorose-producing activity.
Claims1
A biologically pure culture of Torulopsis bombicola KSM-36 (FRI Deposition FERM BP-799) having the following mycological properties:
- (a) state of growth on various culture media:
- grows into elliptical to elongated elliptical Yeasts having a size of (0.75 to 3.0).times.(1.5 to 5.5) .mu.m on malt culture medium, malt agar, potato dextrose agar, and corn meal agar, and no formation of euhyphae or pseudohyphae is observed by the multipolar budding type;
- (b) formation of ascospores:
- using malt extract agar, no formation of ascosporres is observed;
- (c) formation of ballistosxpores:
- culturing on malt agar, no formation of ballistospores is observed;
- ______________________________________ (d) various physiological properties:
- (1) growth conditions:
- pH:
- 3 to 8 (optimum pH:
- 5 to 6) temperature:
- 7 to 38.degree. C. (optimum temperature:
- 25 to 34.degree. C.) (2) assimilation of salts (-) of nitric acid:
- (3) decomposition of fat:
- (+) (4) liquefaction of gelatin:
- (-) (6) resistance to sodium chloride:
- 6-8% (w/v) (7) formation of carotenoid pigments:
- (-) (8) formation of organic acids in (+) Caster's culture medium:
- (9) formation of starch-like (-) substances:
- (10) vitamin requirement:
- (+) (e) assimilating capacity and fermenting capacity:
- assimilating fermenting capacity capacity (1) D-glucose (+) (+) (2) trehalose (-) (-) (3) D-galactose (-) (-) (4) lactose (-) (-) (5) sucrose (+) (+) (6) raffinose (-) (+.sup.1/3) (7) D-mannose (-) (-) (8) D-xylose (-) (9) .alpha.-methyl-D-glucoside (-) (10) melibiose (-) (11) D-arabinose (-) (12) esculin (-) (13) ethanol (+) (14) inositol (-) (15) maltose (-) (-) (16) melezitose (-) (17) glycerin (+) (18) cellobiose (-) (19) inulin (-) (20) D-mannitol (+) (21) soluble starch (-) (22) D-sorbitol (+) and, (f) productivity of sophorose:
- high sophorose producing activity. ______________________________________
20 more elements
Dependencies are inferred from the claim text.