Monoclonal antibodies to legionella, a process for their preparation and their use in the determination of legionella pneumophila

A monoclonal antibody, produced by the murine hybrid cell line deposited in the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur - Paris (France) under No. I-220, which antibody is of the .gamma.-3 isotype.

A monoclonal antibody produced by the murine hybrid cell line deposited in the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur-Paris (France) under No. I-219, which antibody is of the .gamma.-2b isotype.

A diagnostic kit useful in the detection of Legionella pneumophila bacteria which is present in a biological sample using an immunoenzymatic method or immunfluorescence, which contains as the immunological reactant, a monoclonal antibody in an amount suitable for detecting the presence of Legionella pneumophila bacteria, said monoclonal antibody to Legionella produced by a hybrid cell line obtained by a process which comprises fusing splenocytes from mice which have been immunized with bacteria of the family Legionella with non-secreting myeloma cells derived from mice, wherein said antibody is produced by the murine hybrid cell line deposited in the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur-Paris (France) under No. I-220, which antibody is of the .gamma.-3 isotype and diagnostically acceptable components for said detection.

A diagnostic kit useful in the detection of Legionella pneumophila bacteria which is present in a biological sample using an immunoenzymatic method or immunofluorescence, which contains as the immunological reactant, a monoclonal antibody in an amount suitable for detecting the presence of Legionella pneumophila bacteria, said monoclonal antibody to Legionella produced by a hybrid cell line obtained by a process which comprises fusing splenocytes from mice which have been non-secreting myeloma cells derived from mice, wherein said antibody is produced by the murine hybrid cell line deposited in the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur Paris-(France) under No. I-219, which antibody is of the .gamma.-2b isotype and diagnostically acceptable components for said detection.

A vaccine composition useful in combatting the effects of disease caused by Legionella bacteria comprising a monoclonal antibody to Legionella produced by a hybrid cell line obtained by a process which comprises fusing splenocytes from mice which have been immunized with bacteria of the family Legionella with non-secreting myeloma cells derived from mice, wherein said antibody is produced by the murine hybrid cell line deposited in the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur - Paris (France) under No. I-220, which antibody is of the .gamma.-3 isotype and a pharmaceutically acceptable carrier therefor.

A vaccine composition useful in combatting the effects of disease caused by Legionella bacteria comprising a monoclonal antibody to Legionella produced by a hybrid cell line obtained by a process which comprises fusing splenocytes from mice which have been immunized with bacteria of the family Legionella with non-secreting myeloma cells derived from mice, wherein said antibody is produced by the murine hybrid cell line deposited in the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur-Paris (France) under No. I-219, which antibody is of the .gamma.-2b isotype and a pharmaceutically acceptable carrier therefor.