Process for the culture of Babesia canis, application to the preparation of antigenes and vaccines and antigenes and vaccines against pyroplasmosis

Abstract

In a process for the culture of Babesia canis, erythrocytes infected by the Babesia canis parasite are incubated in a culture medium suitable for the survival of the erythrocytes and for breeding of the parasites, in the presence of a homologous serum, in the substantially static state, in a normal atmosphere, first of all at a high temperature of 34.degree. to 38.degree. C., then a low temperature, more especially between 0.degree. and 10.degree. C., this cycle being able to be reproduced. The supernatant product is collected which is centrifugated and filtered. A vaccine is prepared against pyroplasmosis in dogs from this floating medium.

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