Blot overlay assay using colloidal metal particles
AssigneeJanssen Pharmaceutica NV
InventorsMarc K. J. Moeremans, Guido F. T. Daneels, Jan R. De Mey
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Abstract
A method for detecting and/or determining an agglomerate formed by the reaction between a specific binding agent and the corresponding acceptor substance according to the general methodology of blot overlay assays by using colloidal metal particles labelled components which may be visualized as a coloured signal at the surface of the blotting medium characteristric for the colloidal metal particles used or quantitatively determined following art-known spectrophotometric procedures such as densitometry.
Claims14
A process for detecting or determining an acceptor substance in a blot overlay assay comprising the steps of:
- (i) immobilizing the acceptor substance to a blotting medium;
- (ii) contacting said blotting medium with colloidal metal particle labelled specific binding agent for said acceptor substance;
- and
- (iii) detecting the colored signal on the surface of the blotting medium caused by the colloidal metal particles at the reaction site between said acceptor substance and said specific binding agent.
A process for detecting or determining an acceptor substance in a blot overlay assay comprising the steps
- (i) immobilizing the acceptor substance to a blotting medium;
- (ii) contacting said blotting medium with an unlabeled specific binding agent for said acceptor substance and, subsequently, colloidal metal labelled protein specific for the unlabeled specific binding agent;
- and
- (iii) detecting the colored signal on the surface of the blotting medium caused by the colloidal metal particles at the reaction site between said acceptor substance and said specific binding agent.
Dependencies are inferred from the claim text.