US 4,774,322 A · Polypeptide cartilage-inducing factors found in bone

Abstract

Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Claims1

Dependent claims

A polypeptide that:

(a) is found in mammalian bone;
(b) is a co-factor for inducing cartilage formation;
(c) is active in the TGF-.beta. assay;
(d) is a dimer having a molecular weight of approximately 26,000 daltons as determined by SDS-PAGE, whose chains each have the following N-terminal sequence:
Ala-Leu-Asp-Ala-Ala-Tyr-Cys-Phe-Arg-Asn-Val-Gl[u]n-Asp-Asn-Cys-Cys-Leu-Arg -Pro-Leu-Tyr-Ile-Asp-Phe-Lys-Arg-Asp-Leu-Gly-Trp-;
and
(e) is substantially pure, and polypeptides that are substantially pure and substantially equivalent to said polypeptide.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

07/129,864Filed 10 Dec 1987 · continuation

Priority

EP 58.304848.68 Jul 1985

Granted

27 Sep 1988

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

B64C 11/00
Propellers
A61K 35/32
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61L 27/227
Polypeptides or derivatives thereof , e.g. degradation products: other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or…
B65B 35/50
Arranging and feeding articles in groups: stacking one article, or group of articles, upon another before packaging

Polypeptide cartilage-inducing factors found in bone

Life of this patent

Polypeptide cartilage-inducing factors found in bone

Life of this patent

Abstract

Claims1