US 4,774,228 A · Polypeptide cartilage-inducing factors found in bone used in tissue proliferation

Abstract

Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Claims2

Dependent claims

A method of promoting proliferation of soft tissue cells in an animal comprising administering a composition comprising

(a) a polypeptide that
(i) is active in the TGF-b assay;
and
(ii) is a dimer having a molecular weight of approximately 26,000 daltons as determined by SDS-PAGE, whose chains each have the following N-terminal sequence:
Ala-Leu-Asp-Ala-Ala-Tyr-Cys-Phe-Arg-Asn-Val-Gln-Asp-Asn-Cys-Cys-Leu-Arg-Pr o-Leu-Tyr-Ile-Asp-Phe-Lys-Arg-Asp-Leu-Gly-Trp-;
(b) a TGF-b activating agent;
and
(c) a pharmaceutically acceptable carrier, said polypeptide and activating agent being present in amounts that promote normal cell proliferation to the animal.

5 more elements

Dependencies are inferred from the claim text.

Polypeptide cartilage-inducing factors found in bone used in tissue proliferation

Life of this patent

Polypeptide cartilage-inducing factors found in bone used in tissue proliferation

Life of this patent

Abstract

Claims2