Modified vaccinia virus and methods for making and using the same
AssigneeHealth Research, Inc.
InventorsEnzo Paoletti, Dennis Panicali
Life of this patent
Estimating the term…
Abstract
What are disclosed are methods for modifying the genome of vaccinia virus to produce vaccinia mutants, particularly by the introduction into the vaccinia genome of exogenous DNA; modified vaccinia prepared by such methods; certain DNA sequences and unmodified and genetically modified microorganisms involved as intermediates in such methods; and methods for infecting cells and host animals with such vaccinia mutants to provoke the amplification of exogenous DNA and proteins encoded by the exogenous DNA, including antigenic proteins, by said cells and host animals.
Claims19
A method for the in vivo recombination of vaccinia virus DNA with DNA not naturally occurring in vaccinia virus, which method comprises infecting a cell with vaccinia virus in a cell-compatible medium in the presence of doner DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus flanked by DNA sequences homologous with portions of the vaccinia genome, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
A method for the in vivo recombination of vaccinia virus DNA and DNA not naturally occurring in vaccinia virus, which method comprises infecting a cell with vaccinia virus in a cell-compatible medium in the presence of donor DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus present within a segment of vaccinia DNA, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
A method for the in vivo recombination of vaccinia virus DNA with DNA not naturally occurring in vaccinia virus, which method comprises contacting a cell monolayer with a cell-compatible medium containing vaccinia virus and with a cell-compatible medium comprising donor DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus present within a non-essential region of a segment of vaccinia virus otherwise co-linear with portions of the vaccinia genome, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
The method of replicating DNA in a eukaryotic cell by infecting said cell with a vaccinia virus modified to contain said DNA, said DNA not naturally occurring in vaccinia virus.
Dependencies are inferred from the claim text.