Antidigoxin antibodies
AssigneeHSC Research Development Corporation
InventorClifford A. Lingwood
Life of this patent
Estimating the term…
Abstract
A digoxin derivative/immunogenic protein conjugate is disclosed which has the carbohydrate moiety of digoxin intact. Antibodies raised against this conjugate show minimal cross-reactivity to digoxin metabolites enabling the use as an antibody in the diagnostic analysis for digoxin when measured in the presence of its metabolites found in serum isolated from a human.
Claims29
A digoxin derivative/immunogenic protein conjugate, said digoxin derivative having a lactone ring at C17 of digoxin opened at a double bond between C20 and C23 of said lactone ring, said digoxin derivative having the same carbohydrate moiety as digoxin, said immunogenic protein being coupled to the C23 of said opened lactone ring without altering said carbohydrate.
A process for preparing a digoxin derivative/immunogenic protein conjugate comprising opening the double bond between C20 and C23 of the C17 lactone ring of digoxin having a carbohydrate moiety at the C3 of the digoxin molecule, and coupling an immunogenic protein to the C23 of the resultant opened lactone ring without altering said carbohydrate moiety.
Dependencies are inferred from the claim text.