Recombinant DNA which codes for interleukin-1 B
AssigneesNew England Medical Center, Tufts College, Wellesley College, Mit
InventorsPhilip E. Auron, Charles A. Dinarello, Andrew C. Webb, Alexander Rich, Sheldon M. Wolff
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Abstract
The subject invention concerns a nucleic acid comprising a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Specifically, the subject invention comprises the cloning of a cDNA synthesized by reverse transcription of poly(A)RNA isolated from adherent human monocytes stimulated with bacterial endotoxin. Human IL-1 is useful to induce the production of IL-2 by activated T-cells; it also acts on B-cells and NK-cells.
Claims24
A recombinant DNA cloning vehicle comprising a DNA sequence comprising the human IL-1 gene DNA sequence.
Essentially pure DNA coding for human IL-1B having the sequence:
- ##STR29##
Recombinant plasmid pcD-415.
The microorganism E. coli HB101 (pcD-415).
A process for preparing human IL-1B which comprises culturing a microbe hosting a cloning vehicle comprising DNA encoding human IL-1B and recovering human IL-1B.
Essentially pure DNA coding for human IL-1B having the sequence:
- ##STR30##
A recombinant DNA cloning vehicle comprising a DNA sequence comprising cDNA coding for human IL-1b having the sequence:
- ##STR31##
A process for preparing a human IL-1B peptide which comprises culturing a microbe hosting a cloning vehicle comprising DNA having the following sequence:
- ##STR35## and equivalent nucleotide sequences thereof coding for the amino acid sequence:
- ##STR36##
Recombinant plasmid pcD-1218.
Recombinant plasmid pA-26.
Dependencies are inferred from the claim text.