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US 4,762,915 AGranted
Protein-liposome conjugates
AssigneeLiposome Technology, Inc.
InventorsViola T. Kung, Carl T. Redemann
Life of this patent
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Abstract
A lipid coupling reagent for use in coupling amine-containing molecules, such as proteins, to liposomes. The reagent includes phosphatidylethanolamine coupled to a 3-20 atom carbon-containing spacer arm terminating at a carboxyl or thiocarboxyl group. Also disclosed are liposomes prepared to include between about 1 and 20 mole percent of the coupling reagent, and liposomes containing surface arrays of proteins attached to the liposomes through the coupling reagent.
Claims2
A method of attaching protein molecules to liposomes at a concentration of at least about 150 .mu.g protein per .mu.mole lipid, comprising
- providing a carboxylated lipid coupling reagent of the form ##STR11## where PE--NH.sub.2 is phosphatidylethanolamine and CH.sub.2 - - - CH.sub.2 is a carbon-containing chain having a total chain length between 3 and about 20 atoms
- preparing liposomes containing between about 1 and 20 mole percent of the coupling reagent,
- activating the liposomes with a water-soluble carbodiimide, and
- reacting the activated liposomes with such protein, at a protein concentration of at least about 300-500 .mu.g/.mu.mole liposome lipid.
1 more element
Dependencies are inferred from the claim text.