In vivo cellular tracking
AssigneeSmithKline Beckman Corporation
InventorsPaul K. Horan, Sue E. Slezak
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Abstract
Methods of tracking cells in vivo and for determining in vivo cell lifetimes. Cells are labelled with cyanine dyes and detection is by measuring fluorescence, absorbance, or by detecting nuclear magnetic resonance probes included in the cyanine dyes. Using the invented methods, for example, red blood cell and platelet lifetimes are determined. Also, cells are tracked to determine sites of primary or metastatic tumors, or sites of occult infection. Further, rates at which cells pass through vessels is used to determine blood vessel patency and platelet aggregation.
Claims40
A method for tracking cells in vivo in a subject that comprises determining the location of cyanine dye-labelled cells previously administered to the subject.
A method for determining in vivo cell lifetime in a subject that comprises measuring the rate at which cyanine dye-labelled cells administered to the subject disappear from said subject.
A method for tracking cells in vivo in a subject that comprises:
- obtaining cells to be cyanine dye-labelled,
- labelling the cells with a cyanine dye,
- administering the cyanine dye-labelled cells to the subject, and
- detecting the cyanine dye-labelled cells.
Dependencies are inferred from the claim text.