US 4,762,413 A · Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light

Abstract

A coherent laser light flux is projected into a cell made of transparent quartz and light scattered from particles suspended in an antigen-antibody reaction liquid contained in the cell is detected by a photomultiplier by means of a collimator. An output electrical signal from the photomultiplier is sampled at different time instances and samplings are supplied to a fast Fourier transformer to derive a plurality of power spectrum densities of fluctuation in intensity of the scattered light. A plurality of power spectrum densities are averaged to generate a mean power spectrum density. An amount of antigen contained in the reaction liquid is measured in accordance with the mean power spectrum density.

Claims25

Dependent claims

A method of measuring immunological reaction comprising the steps of:

projecting radiation to a reaction liquid containing at least antigen and antibody;
detecting radiation scattered by particulate substances in the reaction liquid;
deriving a plurality of power spectrum densities of fluctuation in intensity of said scattered radiation;
deriving a mean power spectrum density in accordance with said plurality of power spectrum densities;
and
measuring antigen-antibody reaction on the basis of said mean power spectrum density.

3 more elements

An apparatus for measuring an immunological reaction comprising:

a light source for emitting a light flux;
a cell for containing an antigen-antibody reaction liquid;
optical means for projecting the light flux emitted from the light source into said cell;
detecting means for receiving light scattered by particulate substance included in said antigen-antibody reaction liquid and for deriving a plurality of power spectrum densities of fluctuation in intensity of said scattered light;
means for deriving a mean power spectrum density from said plurality of power spectrum denisities;
and
means for measuring an immunological reaction in the antigen-antibody reaction liquid on the basis of the mean power spectrum density.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

06/769,965Filed 27 Aug 1985

Priority

JP 59-1862827 Sep 1984

Granted

9 Aug 1988

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

G01N 21/51
Within a body or fluid: inside a container, e.g. in an ampoule
G01N 21/253
Colorimeters; Construction thereof: for batch operation, i.e. multisample apparatus
G01N 21/82
By observing the effect on a chemical indicator: producing a precipitate or turbidity
G01N 33/53
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…

Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light

Life of this patent

Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light

Life of this patent

Abstract

Claims25