Glucose electrode and method of determining glucose
AssigneeNova Biomedical Corporation
InventorsChung C. Young, Kenneth Gary, Handani Winarta, Chin-Chun Chen
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Abstract
An electrode assembly for polarographic assay of glucose in solution having a laminated membrane, the outer membrane being from 1 to 20 .mu.m thick and having a pore size of 10 to 125 .ANG., the inner membrane having a thickness of 2 to 4 .mu.m, and bonded to the outer membrane with immobilized glucose oxidase. Rapid assays are carried out by measuring the current difference before equilibrium is attained and at least 5 seconds after initial contact of sample with the outer membrane.
Claims10
An electrode assembly for use in a polarographic cell for assay of glucose in solution, said assembly comprising a reference electrode and a sensor electrode having a laminated membrane covering the solution-contacting face of said sensor electrode,
- in which said laminated membrane comprises
- an outer solution-contacting membrane from 1 to 20 .mu.m thick having a pore size of 10 to 125 .ANG.,
- an inner membrane adjacent the face of said sensor electrode from 2 to 4 .mu.m thick, and
- an adhesive glucose oxidase layer between said membranes holding them together,
- said inner membrane having a permeability sufficiently great to ensure passage of hydrogen peroxide from said glucose oxidase layer to the face of said sensor electrode.
Dependencies are inferred from the claim text.