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US 4,757,012 AGranted
Ascorbic acid intermediates and process enzymes
AssigneeGenentech, Inc.
InventorsDavid A. Estell, Robert A. Lazarus, David R. Light, Jeffrey V. Miller, William H. Rastetter
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Abstract
Recombinant plasmids containing the gene encoding 2,5-diketogluconic acid reductase are prepared and used to transform microorganisms. 2,5,DKG reductase is expressed by the microorganisms.
Claims5
A process for producing a recombinant microorganism capable of converting glucose to 2-KLG which comprises transforming a host cell capable of converting glucose to 2,5-DKG but lacking the ability to convert 2,5-DKG to 2-KLG with an expression vector which encodes 2,5-DKG reductase.
A recombinant plasmid comprising a DNA segment encoding the enzyme 2,5 DKG reductase.
Dependencies are inferred from the claim text.