Recombinant DNA having a phosphoenol pyruvate carboxylase gene inserted therein, bacteria carrying said recombinant DNA and a process for producing amino acids using said bacteria
AssigneeAjinomoto Co., Inc.
InventorsKonosuke Sano, Koichi Ito, Kiyoshi Miwa, Shigeru Nakamori
Life of this patent
Estimating the term…
Abstract
A recombinant DNA molecule comprising a plasmid vector having operationally inserted therein a gene coding for phosphoenol pyruvate carboxylase is disclosed along with bacteria containing this recombinant DNA molecule and methods of using these bacteria to produce amino acids in large quantities.
Claims7
A recombinant DNA molecule comprising a plasmid and a gene coding for phosphoenol pyruvate carboxylase operationally inserted therein, wherein said recombinant DNA molecule is capable of progagating and said gene is capable of being expressed in a Corynebacterium and Brevibacterium and wherein said gene is a chromosomal gene isolated from a Coryneform strain or a Brevibacterium strain carrying a phosphoenol pyruvate carboxylase, PEPC, gene.
A Corynebacterium or Brevibacterium carrying a recombinant DNA molecule comprising a plasimid having operationally inserted therein a gene coding for phosphoenol pyruvate carboxylase, said gene being a chromosomal gene isolataed from a Coryneform strain or a Brevibacterium strain carrying a PEPC gene, wherein said Corynebacterium or Brevibacterium carrying the recombinant DNA molecule expresses both said gene and a chromosomal gene coding for an amino acid.
A process for producing an amino acid by fermentation, which comprises:
- cultivating in a culture medium a Corynebacterium or Brevibacterium (1) carrying a recombinant DNA molecule comprising a plasmid having operationally inserted therein a gene coding for phosphoenol pyruvate carboxylase, said gene being a chromosomal gene isolated from a Coryneform strain or a Brevibacterium strain carrying a PEPC gene and (2) having a chromosomal gene coding for said amino acid, and
- isolating said amino acid from said culture medium.
Dependencies are inferred from the claim text.