Human factor VIII:C gene and recombinant methods for production
AssigneeGenetics Institute, Inc.
InventorsJohn J. Toole, Jr., Edward Fritsch
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Abstract
The protein having factor VIII:C procoagulant activity has been produced by culturing a cell transformed with a recombinant expression vector encoding the gene for that activity.
Claims8
An isolated recombinant vector containing DNA coding for human factor VIII:C, comprising a polydeoxyribonucleotide having the sequence:
- ##STR2##
A non-human recombinant expression vector for human factor VIII:C comprising a DNA segment having the sequence:
- ##STR3## operatively linked to a heterologous regulatory control sequence.
A screening agent for identifying deoxyribonucleotide sequences and ribonucleotide sequences which encode for at least a portion of the human gene factor VIII:C, consisting essentially of a deoxyribonucleotide having the following sequence or its inverse complement:
- ##STR4##
A method of producing a protein having factor VIII:c procoagulant activity, comprising the steps of:
- (1) culturing an isolated cell transformed with double stranded DNA which DNA
- (a) has been operatively linked to a heterologous regulatory control sequence,
- (b) hybridizes under stringent conditions to the following nucleotide sequence:
- ##STR5## and (c) encodes a protein which upon expression exhibits human factor VIII:c procoagulant activity, and
- (2) recovering said protein from the culture.
A transformed non-human mammalian cell containing a gene encoding human factor VIII:c in operable association with a heterlogous regulatory control sequence in an expression vector therefor.
Dependencies are inferred from the claim text.