Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
AssigneeGenentech, Inc.
InventorsGregory L. Gray, Herbert L. Heyneker
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Abstract
The production of mature hGH In E. coli and Pseudomonas strains transformed by a plasmid which encodes pre hGH (comprising the signal polypeptide and the hormone itself) is described. These prokaryotes process the pre-hGH to cleave the signal sequence and, thereby, produce mature hGH.
Claims2
A method comprising (a) transforming a procaryotic cell with an expression plasmid encoding a pre protein for human growth hormone containing a signal sequence, culturing the transformed cell to provide a prokaryotic recombinant host cell culture comprising mature human growth hormone, which growth hormone is free of mature human growth hormone having an extraneous N-terminal methionine, and (b) isolating the mature human growth hormone from a cell extract of the host cell culture of step (a).
Dependencies are inferred from the claim text.