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US 4,753,886 AGranted
Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes
AssigneeEli Lilly and Company
InventorsCharles L. Hershberger, Jeffrey L. Larson
Life of this patent
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Abstract
The present invention discloses selectable, recombinant DNA shuttle vectors for use in streptomycetes and E. coli. The shuttle vectors of the present invention are present at moderately high copy number. The invention further discloses transformants of the aforementioned vectors.
Claims33
A moderately high copy number recombinant DNA cloning vector comprising:
- (a) the origin of replication-containing KpnI restriction fragment approximately 2.5 kb in size of plasmid pJL192,
- (b) one or more DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive restrictionless host cell;
- (c) said vector capable of replicating to a moderately high copy number in said host cells.
A recombinant DNA cloning vector selected from the group consisting of plasmids pHJL200 , phJL202, pHJL203, pHJL204 and pHJL205.
A DNA molecule consisting of the approximately 2.5 kb Kpn I fragment of pJL192.
Dependencies are inferred from the claim text.