Method of determining major and minor peaks in a chromatogram using a data processor
AssigneeHitachi, Ltd.
InventorTouhachi Yoshihara
Life of this patent
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Abstract
A data processor for chromatography operates to separately recognize the peaks of a composite peak of a chromatogram. At first, in order to separate the peaks, the maximum point of the peak of the chromatogram is determined, and extrapolation lines are drawn from said maximum point to the individual points of the chromatogram, and then the gradients of the lines changing from the minimum to the maximum are determined. By determining the changes of the gradients of the extrapolation lines and intersection points of the extrapolation lines and the chromatogram, the major and minor peak are separated so that the shoulder peak can be judged remarkably accurately.
Claims6
A method of determining major and minor peaks in a chromatogram using a data processor having a storage device for storing data and processing unit responsive to stored programs for processing data stored in said storage device, comprising the steps of:
- (a) storing in a time sequence data representing the successive points of a chromatogram of a sample in said storage device, said chromatogram having at least one peak;
- (b) detecting the maximum value of the data stored in said storage device and the location of the point having said maximum value in said time sequence by means of said processing unit so as to determine the location of said point of maximum value;
- (c) calculating the gradient of each of a plurality of lines which extend from said point of maximum value through respective points of said chromatogram located in said time sequence prior to said point of maximum value by using said processing unit;
- and
- (d) detecting whether both major and minor peaks occur in said chromatogram and determining the location of the minor peak by analyzing said detected gradients using said processing unit.
A method of determining major and minor peaks in a chromatogram using a data processor having a storage device for storing data and a processing unit responsive to stored programs for processing data stored in said storage device, comprising the steps of:
- (a) storing in a time sequence data representing the successive points of a chromatogram of a sample in said storage device, said chromatogram having at least one peak;
- (b) detecting the maximum value of the data stored in said storage device and the location of the point having said maximum value in said time sequence by means of said processing unit so as to determine the location of said point of maximum value;
- (c) calculating the gradient of each of a plurality of lines which extend from said point of maximum value through respective points of said chromatogram located in said time sequence subsequent to said point of maximum value by using said processing unit;
- and
- (d) detecting whether both major and minor peaks occur in said chromatogram and determining the location of the minor peak by analyzing said detected gradients using said processing unit.
Dependencies are inferred from the claim text.