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US 4,751,190 AGranted
Fluorescence polarization immunoassay and reagents for use therein
AssigneeAbbott Laboratories
InventorsEnrico G. Chiapetta, Robert J. Kucera
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Abstract
An method of carrying out a fluorescence polarization immunoassay is disclosed wherein the immunoassay is conducted in the presence of from about 0.001 to about 1.0 percent (weight/volume) of dioctyl sodium sulfosuccinate. Also disclosed are reagents useful in the practice of the immunoassay method.
Claims3
In a method of determining the presence or amount of a ligand in a sample by fluorescence polarization immunoassay comprising adding a tracer and an antibody specific for said ligand and observing a polarization value as an indication of the amount of ligand in the sample the improvement comprising:
- conducting the fluorescent polarization immunoassay in the presence of from about 0.001 to about 1.0 percent (weight/volume) of dioctyl sodium sulfosuccinate.
Dependencies are inferred from the claim text.