Method and apparatus for the submerged growth of cell cultures
AssigneeChemap AG
InventorsDaniel Karrer, Henry S. Bondi
Life of this patent
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Abstract
In a method and apparatus for the submersed growth of human and animal cell cultures, the cell growth container is provided with a centrally disposed permeator, which is formed simultaneously as conduit tube and reactor, and is provided on both sides with gas-permeable membranes. A gas of defined composition is led through the hollow porous support body of the permeator as fresh gas on one side, and led away on the other side as waste gas. In this manner the gas delivers oxygen across the membrane and receives carbon dioxide. The nutrient substrate and the tissue cells are held in suspension by means of a stirrer provided in the interior of the permeator. Foam problems do not occur, since no gas bubbles can enter into the medium.
Claims11
Method for the submersed growth of tissue culture cells in a cell growth apparatus comprising a container, a stirrer located in the container, and an annular porous support body having interior and exterior sides and an interior and having cleanable gas-permeable membranes of a thickness no greater than about 0.1 mm on the interior and exterior sides of the support body, said support body located within the container,
- said method comprising
- introducing tissue culture cells and nutrient solution into the container,
- introducing oxygen into the interior of the support body,
- and cultivating the tissue culture cells by stirring the container contents to circulate the tissue culture cells and nutrient solution in the container while diffusing oxygen from the support body interior across the membranes to the nutrient solution and diffusing CO.sub.2 from the nutrient solution across the membranes to the support body interior.
Cell growth apparatus comprising
- container means for containing tissue culture cells and nutrient solution;
- stirring means for circulating the tissue culture cells and the nutrient solution within the container;
- permeator means located within the container means for diffusing oxygen into the nutrient solution and for removing CO.sub.2 from the nutrient solution, said permeator means including an annular porous support body having interior and exterior sides and an interior and having cleanable gas-permeable membranes of a thickness no greater than about 0.1 mm on the interior and exterior sides of the support body;
- supply means for introducing oxygen into the interior of the support body;
- and
- removal means for removing carbon dioxide from the interior of the support body.
Dependencies are inferred from the claim text.