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US 4,748,124 AGranted
Compartmentalized cell-culture device and method
AssigneeE. I. Du Pont de Nemours and Company
InventorErwin A. Vogler
Life of this patent
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Abstract
A closed, cell culture device constructed using a first and second sheet of gas permeable, liquid-impermeable material. A third sheet of a material selectively permeable to a class of molecules sandwiched between the first and second sheets, all of the sheets being formed such that the first and third sheets define a first closed compartment and the second and third sheets define a second closed compartment, each compartment having an access port.
Claims9
A method of culturing cells comprising the steps:
- placing the cells in a first compartment with a culture medium, said first compartment composed of an upper dialysis membrane and a lower gas-permeable, liquid-impermeable sheet;
- injecting a culture medium in a second compartment above said dialysis membrane;
- permitting waste products from said cells to flow upwardly through said dialysis membrane;
- and
- exchanging incubator gases through said gas-permeable sheet whereby said cells grow continuously without clogging said dialysis membrane.
2 more elements
A closed dual compartment cell culture device comprising:
- two sheets of gas-permeable, liquid impermeable material disposed horizontally with one over the other to form a top sheet and a bottom sheet;
- a dialysis membrane disposed between said two sheets;
- one separating means between said membrane and said bottom sheet to form a bottom compartment adapted to contain cells to be cultured and a culture medium;
- second separating means between said membrane and said top sheet to form a top compartment adapted to contain a culture medium;
- access ports in each of said separating means to permit injection of material into said compartments;
- said dialysis membrane adapted to permit waste products to flow from said bottom compartment to said top compartment while adapted to retain macromolecules for cell growth in the bottom compartment and said gas-permeable sheets are adapted to exchange incubator atmospheric gases.
3 more elements
Dependencies are inferred from the claim text.