Method for forming amphotericin B crystals in fermentation broth
AssigneeE. R. Squibb & Sons, Inc.
InventorsRaphael Y. Ko, Laszlo J. Szarka
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Abstract
A new method is provided for forming crystals of amphotericin B directly in fermentation broth which crystals are easily separable without use of costly solvent extraction techniques which method includes a direct microbial cell autolysis step to induce crystal formation.
Claims13
A method for producing crystals of amphotericin B directly in a fermentation medium, which consists essentially of cultivating a strain of Streptomyces nodosus in a fermentation medium which is free of organic solvent for amphotericin B until substantial antifungal activity is imparted to said medium, heating the medium to a temperature of from about 70.degree. to about 130.degree. C. for at least 10 minutes to form crystals of amphotericin B, and recovering substantial amounts of said crystals of amphotericin B from said medium.
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