Streptomyces secretion vector

A DNA vector for producing a desired protein in a Streptomyces host, said vector comprising the following components:

• (a) a DNA sequence coding for a signal sequence, wherein said sequence is identical to the DNA signal sequence of a Streptomyces endoH gene, or an engineered or chemically synthesized variant thereof;
• (b) an engineered restriction site positioned to receive DNA that codes for a protein to be secreted and that is not naturally translated and transcribed with said DNA coding for a signal sequence wherein said restriction site is operably linked to said DNA signal sequence;
• and
• (c) regulator DNA that includes a promoter sequence effective to start tanscription in said host Streptomyces, wherein said promoter is positioned and oriented in said vector to initiate transcription toward the signal sequence-encoding DNA and the structural gene.

A host Streptomyces bacterial cell from a species other than S. plicatus, said host cell comprising an expression vector comprising:

• (a) a DNA sequence coding for a signal sequence, said DNA sequence being identical to a DNA sequence that occurs in the endoH gene of S. plicatus or an engineered or chemical derivative thereof;
• (b) a structural gene that codes for a desired protein wherein said gene is operably linked to said DNA signal sequence;
• and
• (c) regulatory DNA that includes a promoter sequence effective to start transcription in said host Streptomyces, said promoter being positioned and oriented in said vector to initiate transcription toward the signal sequence-encoding DNA and the structural gene.