Method of preparing liposomes and products produced thereby
AssigneeE. R. Squibb & Sons, Inc.
InventorsNicholas I. Payne, Peter Timmins, Cheryl V. Ambrose
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AssigneeE. R. Squibb & Sons, Inc.
InventorsNicholas I. Payne, Peter Timmins, Cheryl V. Ambrose
Estimating the term…
A method is provided for preparing stable liposome precursors in the form of a water-soluble carrier material coated with a predetermined amount of a thin film of liposome components, which method includes the steps of dissolving at least one liposome-forming amphipathic lipid, optionally, at least one biologically active compound, and, optionally, at least one adjuvant in a suitable organic solvent and employing the resulting organic solution to coat a suitable water-soluble carrier material to form thin film of predetermined amount of liposome components thereon. Upon exposing the coated carrier material to water, the thin films of liposome components hydrate and the carrier material dissolves to give liposome preparations.
A method for preparing a stable liposome precursor in the form of a thin film of liposomal components of predetermined amount coated on a water-soluble particulate carrier material which is suitable for intravenous use, which liposome precursor may be hydrated to form a liposome product of controlled and acceptable mean size, which comprises forming a solution, in an organic solvent, of at least one liposome-forming lipid selected from the group consisting of a phospholipid, a natural lecithin and a synthetic lecithin in an amount of from about 1 to about 25% by weight of the organic solution, optionally, at least one biologically active compound which is medicament, protein, enzyme, hormone or diagnostic agent, and, optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation, and coating a particulate water-soluble carrier material which is suitable for intravenous use and is substantially insoluble in said organic solvent, with the so-formed organic solution to form a thin film of liposomal components of predetermined desired amount on said carrier material which thin film contains a ratio of lipid:optional biologically active compound (where present) of within the range of from about 0.5:1 to about 1000:1, which so-coated particulate water-soluble carrier material may be exposed to a hydration medium to form a final hydrated liposome product of controlled and desired mean size.
A stable liposome precursor which when mixed with water forms a liposome preparation of controlled and acceptable mean size distribution, comprising a water-soluble particulate carrier material suitable for intravenous use coated with a predetermined amount of a thin film of liposomal components comprised of at least one liposome-forming lipid selected from the group consisting of a phospholipid, a natural lecithin and a synthetic lecithin, optionally, at least one biologically active compound which is a medicament, protein, enzyme, hormone or diagnostic agent, and, optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation and which thin film contains a ratio of lipid:optional biologically active compound (where present) of within the range of from about 0 5:1 to about 1000:1.
Dependencies are inferred from the claim text.