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US 4,743,560 AGranted
Solid phase assay
AssigneeBecton, Dickinson and Company
InventorsRobert L. Campbell, Daniel B. Wagner, James P. O'Connell
Life of this patent
Estimating the term…
Abstract
Solid phase assay for an analyte wherein binder is supported on a solid support, such as nitrocellulose, and the tracer is comprised of ligand labeled with a particulate label, such as a liposome, including a detectable marker which is not visible.
Claims34
A process for assaying for an analyte, comprising:
- contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a liposome, said liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder and said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome;
- and
- determining the tracer bound in said test area as a measure of analyte in a sample.
A process for assaying for an analyte, comprising:
- contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labled with a liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of nitrocellulose, said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome;
- and
- determining the tracer bound in said test area as a measure of analyte in a sample.
A reagent kit for determining an analyte comprising:
- a solid support including a binder on a test area of a solid support;
- and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labeled with a liposome containing a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support during an assay, said test area being formed of a material having a surface area supporting the binder and the binder being supported in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome.
A process for assaying for an analyte, comprising:
- contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a lipsome, said liposome including a detectable marker which is not visible, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome;
- and
- determining the tracer bound in said test area as a measure of analyte in a sample.
Dependencies are inferred from the claim text.