Derived nisin producing microorganisms, method of production and use and products obtained thereby
AssigneeMicrolife Technics, Inc.
InventorsCarlos F. Gonzalez, Alfred J. Gryczka
Life of this patent
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Abstract
Derived microorganisms containing foreign DNA encoding for nisin production and a method for producing the derived microorganisms by transferring DNA to a recipient microorganism are described. The recipient microorganisms are preferably bacteria lacking in the ability to produce nisin. Nisin inhibits the growth of spoilage bacteria and is used in various materials for preservation, including foods and particularly at refrigeration temperatures. Nisin is also used in animals for improving the health of the animal. The foreign DNA is obtained from a donor microorganism and encodes for nisin production when transferred to the recipient microorganism.
Claims8
In a method of preserving a material by incorporating a bacterium into the material the improvement which comprises incorporating into the material a nisin producing derived bacterium containing foreign DNA encoding for nisin production derived from a nisin producing donor bacterium and which foreign DNA has been transferred to a recipient bacterium to produce a nisin producing bacterium, wherein the donor bacterium is a biologically pure Streptococcus species containing plasmid DNA derived from a 29 Mdal plasmid encoding for sucrose utilization and nisin production and wherein the recipient bacterium is a biologically pure Streptococcus species which is sensitive to nisin.
An improved method for preservation of a fresh or fermented food product by providing a bacterium in admixture with the food, the improvement which comprises:
- holding the food at refrigeration temperatures in the presence of a nisin producing derived bacterium containing foreign DNA encoding for nisin production derived from a nisin producing donor bacterium and which foreign DNA has been transferred to a recipient bacterium to produce a nisin producing bacterium, wherein the donor bacterium is a biologically pure Streptococcus species containing plasmid DNA derived from a 29 Mdal plasmid encoding for sucrose utilization and nisin production and wherein the recipient bacteria is a biologically pure Streptococcus species which is sensitive to nisin.
Dependencies are inferred from the claim text.